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m, and a rise in the standard volume of Zn sure to the protein, suggesting that a further metallic ion can bind to this mutant. The fall in catalytic exercise may consequence from the binding of this second steel ion or to steric crowding within the confines of the active website which includes evidently progressed to work with an individual divalent cation.
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by using a battery of substrate analogs and active web-site mutants using a host of metal-substituted sorts of the enzyme. We conclude from these scientific studies that NagA from E. coli
a worth is somewhat larger for login naga26 Cd-NagA than for Zn-NagA, as anticipated in the tendency of zinc to lessen the p
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The metal information along with the corresponding kinetic parameters with the metal-substituted variants of NagA are listed in Table 1.
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